Sampling, Preservation and Storage of Water Sample

1.0 Objective
To lay down a procedure for the Sampling, Preservation and Storage of water sample.
2.0 Scope
This Standard Operating Procedure is applicable for Sampling, Preservation and Storage of water sample.
3.0 Responsibility
All Microbiologists/QC officers are responsible to follow the SOP.
Quality Control Head or his/her designee is responsible for compliance of this SOP.
4.0 Accountability
Department Head & QA Head shall be accountable for implementation of this SOP.
5.0 Abbreviations and Definitions
QC      :           Quality Control
SOP     :           Standard Operating Procedure
6.0 Procedure
6.1 Sampling of water for Microbiological analysis
  • Collect water sample for microbiological analysis in clean and sterilized nonreactive borosilicate glass or polypropylene bottles.
  • Neutralized the chlorinated water sample adding 1% sterile sodium thiosulphate solution.
  • Open the sample port/tap fully and allow the water to drain for validated time to remove the contamination in the pipe line.
  • While sampling do not contaminate inner surface of stopper or cap and neck of bottle. Do not touch the edge of the sample container.
  • Fill the required volume and replace the cap immediately.
  • Collect quantity of water samples required for chemical testing in non-relative borosilicate Glass or plastic bottles that have been cleaned and rinsed carefully and given a final rinse with purified water.
  • Before sampling for chemical test rinse the bottle with same sample for at list 3 times, fill the bottle completely without leaving any shape for air and replace the cap immediately.
  • Leave about 1% air space in the bottle sufficient to facilitate mixing by shaking before examination.
6.2 Sampling of water for Chemical analysis and Bacterial Endotoxin testing
  • Sample of water for chemical analysis should be collected in a clean and dried bottle and for Bacterial Endotoxin testing sample should be collected in depyrogenated or pyrogen free vial covered with aluminum foil.
  • Open the sample port/tap fully and allow the water to drain for validated time (approximately 1 minute) to remove the contamination in the pipe line.
  • For TOC analysis collect the sample in the separate vial/bottles.
  • Do not allow the vial or water in the vial to come in the contact of water.
  • Without Overflowing, fill the vial.
  • Close with the cap immediately Chemical analysis sample vial and Bacterial Endotoxin testing sample cover with aluminum after sampling.
  • Close the valve.
  • Fill the information on the face of bottle such as location of sampling, date of sampling with signature.
  • Collect all samples and proceed for analysis as per the SOP.
6.3 Shipment
  • If a contract laboratory is utilized, samples shall be sent by express service to arrive at the contract laboratory.
  • Sample container transfer “boxes” should include provision for maintaining the sample temperature below 25°C at any time and sample should not be frozen or cooled to a temperature 2°C.
6.4 Preservation and Storage
  • It is recommended that sample should be analyzed immediately and storage should be avoided.
  • For microbiological testing analyze the sample within 6 hrs. of sampling.
  • Refrigerate (2to 8°C) the sample until is commenced.
  • Allow the sample to reach ambient temperature prior to testing.
  • Agitate sample for uniform distribution before testing.
7.0 Forms and Records (Annexures)
8.0 Distribution
  • Master copy –           Quality Assurance
  • Controlled copies- Quality Assurance, Quality Control
9.0 Reference:   
If any
10.0 History
Revision Number
                                        Reason for Revision
                                                  New SOP