Sampling, Preservation and Storage Procedure of Water Sample  

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1.0 Objective
To lay down a procedure for the Sampling, Preservation and Storage of water sample.
2.0 Scope
This Standard Operating Procedure is applicable for Sampling, Preservation and Storage of water sample.
3.0 Responsibility
3.1 All Microbiologists/QC officers are responsible to follow the SOP.
3.2 Quality Control Head or his/her designee is responsible for compliance of this SOP.
4.0 Accountability
4.1 Department Head & QA Head shall be accountable for implementation of this SOP.
5.0 Abbreviations and Definitions
QC      :           Quality Control
SOP     :           Standard Operating Procedure
6.0 Procedure
6.1 Collect water sample for microbiological analysis in clean and sterilized nonreactive borosilicate glass or polypropylene bottles.
6.2 Neutralized the chlorinated water sample adding 1% sterile sodium thiosulphate solution.
6.3 Open the sample port/tap fully and allow the water to drain for validated time to remove the contamination in the pipe line.
6.4 While sampling do not contaminate inner surface of stopper or cap and neck of bottle. Do not touch the edge of the sample container.
6.5 Fill the required volume and replace the cap immediately.
6.6 Collect quantity of water samples required for chemical testing in non-relative borosilicate Glass or plastic bottles that have been cleaned and rinsed carefully and given a final rinse with purified water.
6.7 Before sampling for chemical test rinse the bottle with same sample for at list 3 times, fill the bottle completely without leaving any shape for air and replace the cap immediately.
6.8 Leave about 1% air space in the bottle sufficient to facilitate mixing by shaking before examination.
6.1.1 Preservation and Storage
6.1.1.1 It is recommended that sample should be analyzed immediately and storage should be avoided.
6.1.1.2 For microbiological testing analyze the sample within 6 hrs. of sampling.
6.1.1.3 Refrigerate (2 to 8 °C) the sample until is commenced.
6.1.1.4 Allow the sample to reach ambient temperature prior to testing.
6.1.1.5 Agitate sample for uniform distribution before testing.
7.0 Forms and Records (Annexures)
NA
8.0 Distribution
8.1 Master copy –           Quality Assurance
8.2 Controlled copies- Quality Assurance, Quality Control
9.0 History
         Date
   Revision Number
                    Reason for Revision
            –
            –
                            New SOP

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