Correct Pipetting Technique

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During Analysis pipetting is a very important to obtain a precise result, pipetting plays important role during analysis, one drop excess or less of solution can bring a OOS (out of specification).

To follow a correct pipetting technique must be followed by the analyst,

Definition: Pipetting is categorized as a measurable instrument, it is graduated cylinder glass tube, used to transfer the solution from one to next.

During Pipetting following points can be correct pipetting:

  • Use appropriate sized pipettes with respect to the quantity of sample solution to  be pipetted
  • In case 10 ml is to be pipetted, do not use a 25 ml pipette or a 5 ml pipette twice.
  • Pipette out the solution, drain the pipette and observe for any adherence of solution to the inner wall of pipette. If solution adheres to the pipette wall, do not  use such pipettes.
  • Pipette out the solution well above the mark.
  • Wipe the outside surface of pipette with tissue paper.
  • Adjust the meniscus of liquid by holding the pipette vertically and at eye level
  • Please note in case of colourless liquids, the lower meniscus is to be considered and in case of  coloured liquids, the upper meniscus.
  • Transfer the required solution up to the mark. Do not blow out the solution left at the tip of pipette.

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