In pharmaceuticals, pathogen testing is very important and critical testing in which different media are used to identify whether specified pathogen is present in the product or not. This pathogen identification is part of Microbial limit testing (MLT) in which first enrichment of sample is performed and then enriched sample is streaked on different media to identify the pathogens. Pathogens should be absent from the sample as per pharmacopoeia. If pathogen is present in the sample then product is not suitable for use. Different pharmacopoeias ask for testing of different pathogens. Below is the table showing list of pathogens as per IP,BP and USP.
ATCC number and morphological characteristics are also mentioned in this table.
S.No. |
Name of Pathogens to be tested as per different Pharmacopoeia |
ATCC No. |
Morphological characters |
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IP |
BP |
USP |
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1 |
Bile tolerant gram negative bacteria |
Bile tolerant gram negative bacteria |
Bile tolerant gram negative bacteria |
NA |
Gram negative rod shaped |
2 |
Escherichia coli |
Escherichia coli |
Escherichia coli |
8739 |
Gram negative rod shaped |
3 |
Salmonella |
Salmonella |
Salmonella |
14028 |
Gram negative rod shaped |
4 |
Staphylococcus aureus |
Staphylococcus aureus |
Staphylococcus aureus |
6538 |
Gram positive round shaped |
5 |
Pseudomonas aeruginosa |
Pseudomonas aeruginosa |
Pseudomonas aeruginosa |
9027 |
Gram negative rod shaped |
6 |
Clostridia |
Clostridia |
Clostridia |
19404 |
Gram positive drum stick shaped |
7 |
Candida albicans |
Candida albicans |
Candida albicans |
10231 |
Gram negative rod shaped |
8 |
Shigella |
Not mentioned |
Not mentioned |
12022 |
Unicellular yeast |