Analytical Method of Medicinal Product Starting Generic Name with word “B”

0
67
Assay Method of Baclofen  / Tablet
Buffer
Take 1.5 gm Sodium -1-Pentane Sulfonate & 10 ml of Glacial Acetic Acid in a 500ml   volumetric flask and make volume with water.
Ratio
Methanol: Buffer º 60 : 40
Diluents
Take 50 ml Methanol & 10 ml of Glacial Acetic Acid in 500ml volumetric flask and made volume with water for the preparation of diluents.
Standard
                                    200 mg —-® 100 ml (Shake 15 minutes)
Sample
Same as standard.
Condition
Waters Spherisorb 10 mm ODS1  4.6 ´ 250mm./ 40º C / UV at 265 nm / 10 minutes.
Assay Method of Betahistine Didydrochloride / Tablet
Solvent
0.1N HCl
Standard
40 mg —-® 100 ml —-® take 2 ml —-® 50 ml  (Shake 20 minutes)
Sample
Same as standard. Measure the absorbance at 260 nm taking 0.1M Hydrochloride Acid as blank.
Assay Method of Bisprolol Hemifumarate  / Tablet
M. Phase
To a 500 ml portion of diluent add 2.5 ml heptaflurobutyric acid, 2.5 ml of diethyl amine and 1.25 ml formic acid. Mix, filter and degas.
Diluents
Water and Acetonitrile (65:35)
Standard
50 mg —-® 50 ml (Shake 20 minutes & Sonicate 5 minutes)
Sample
Same as standard.
Condition
Waters Spherisorb C8 10 µm 4.6 ´ 250mm./ 40º C / UV at 273 nm / 15 minutes.
Assay Method of Bisacodyl / Tablet
Sample
Shake a quantity of the powder containing 40 mg of Bisacodyl with 70 ml of chloroform for 30 minutes, dilute to 100 ml with chloroform, mix, filter and dilute 10 ml of the filtrate to 100 ml with chloroform. Measure the absorbance of the resulting solution at the maximum at 264 nm using chloroform as blank.
% of content =   Absorbance of Sample x 100 / Concentration of Sample x 148
Assay Method of Bromazepam / Tablet
Solvent
Methanol
Standard
25 mg —-® 100 ml —-® take 1 ml —-® 50 ml  (Shake 15 minutes)
Sample
Same as standard. Measure the absorbance at 234 nm.Use Methanol as blank.
Assay Method of Bromhexine Hydrochloride / Tablet
Solvent
Methanol
Standard
25 mg —-® 50 ml —-® take 2 ml —-® 50 ml  (Shake 15 minutes)
Sample
Same as standard. Measure the absorbance at 249 nm Use Methanol as blank.
Assay Method of Bromocriptin  Mesilate / Tablet
Sample
Weigh and powder 20 tablets. To a quantity of he powder containing 5 mg of Bromocription into a 100 ml volumetric flask, add 70 ml of methanol (50%) , shake for 30 minutes & dilute to 100 ml with the same solvent mixture. Mix well & filter. Measure the absorbance of the supernatant solution at the maximum at 305 nm, taking 144 as the value of A (1%; 1 cm) at the maximum at 305 nm.
Calculation
% of Bromocription =  Absorbance of Sample ´ 100 / Concentration in gm per 100 ml x 144

LEAVE A REPLY

Please enter your comment!
Please enter your name here